ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Hours of tutorials: 1.5 Expository Class: 20 Interactive Classroom: 17 Total: 38.5
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Morphological Science
Areas: Human Anatomy and Embryology
Center Faculty of Biology
Call: First Semester
Teaching: With teaching
Enrolment: Enrollable
- To describe the design and equipment of the cell culture laboratory.
- To know different types of cell cultures and their applications.
- To know the fundamentals and applications of cell therapy and tissue engineering.
- To describe the concept and applications of induced pluripotent cells (iPS).
- To know how to apply the basic techniques of cell culture and those to obtain and manipulate stem cells.
- To know how to describe the techniques of cell transplantation and to evaluate the efficacy of the transplantation.
A) Expository classes (20h)
Thematic block I. Cell cultures (14h)
Theme1. Introduction to cell cultures.
Theme 2. Design and equipment of the cell culture laboratory.
Theme 3. Asepsis and biosafety in the cell culture laboratory.
Theme 4. Basic requirements for animal cell culture.
Theme 5. Biology of cells in culture.
Theme 6. Primary cultures.
Theme 7. Cell lines.
Theme 8. Cell characterization techniques in cell cultures.
Thematic block II. Regenerative medicine: cell therapy and tissue engineering (2h)
Theme 9. Cell therapy and regenerative medicine.
Theme 10. Tissue engineering.
Thematic block III. Obtaining and manipulating stem cells (4h)
Theme 11. Concept of stem cell.
Theme 12. Generation of pluripotent stem cells.
Theme 13. Ethical and legal considerations of stem cell research.
B) Interactive classes
B.1- Seminars (7h)
Activities based on problem-solving and critical reflection on relevant issues in the field of cell culture in biotechnology and regenerative medicine will be carried out. Students will also work in teams on current issues related to the subject.
B.2- Laboratory classes (10h)
P1. Basic cell culture techniques.
P2. Characterization of cells in culture I. Viability tests and determination of cell phenotype.
P3. Characterization of cells in culture II. Calculation of parameters indicating the behaviour of cells in culture.
P4. Morphological and functional evaluation of transplants in cell therapy.
Basic
Atala, Anthony and Lanza, Robert, eds., 2014. Essentials of stem cell biology. 3th ed. New York: Elsevier Academic Press.
Atala, Anthony, Lanza, Robert, Mikos, Tony and Nerem, Robert, eds., 2019. Principles of regenerative medicine. 3rd ed. New York: Academic Press-Elsevier.
Davis, John M., ed., 2011. Animal cell culture. Essential methods. Chichester: Wiley-Blackwell. Electronic access.
Freshney, R. Ian, 2016. Culture of animal cells: A manual of basic technique and specialized applications. 7th ed. New Jersey: Wiley Blackwell. Electronic access.
Helgason, Cheryl D. and Miller, Cindy L., eds., 2013 Basic cell culture protocols, 4th ed. New York: Humana Press.
Sell, Stewart, ed., 2013. Stem cells handbook. 2th ed. New Jersey: Springer Humana Press.
Slack, Jonathan M.W., 2018. The science of stem cells. Hoboken: John Wiley. Electronic access.
Complementaria
Clarke, Mary L. and Frampton, Jonathan, 2020. Stem cells: Biology and application. Boca Raton: CRC Press.
Lo Celso, Cristina, Red-Horse, Kristy, Watt and Fiona M., eds., 2020, Stem cells: From biological principles to regenerative medicine. New York: Cold Spring Harbor Laboratory Press.
Rodrigues, Gabriela and Roelen, Bernard, eds., 2020. Concepts and applications of stem cell biology: A guide for student. New Jersey: Springer.
Scientific papers and other resources of interest may be used for topics or activities. If necessary, faculty will facilitate access to these resources through the virtual classroom.
Knowledge
Con01: To know the most important concepts, methods and results of the different branches of Biotechnology.
Con06: To know the fundamentals of cell and tissue bioengineering, the therapeutic possibilities of regenerative medicine and its current technical and ethical limitations.
Skills and abilities
H/D02: Search for, process, analyse/interpret and synthesise relevant information and results from different sources and draw conclusions on biotechnology-related issues.
H/D05: Work in a team.
H/D10: Knowing how to analyse data and interpret experimental results specific to the fields of Biotechnology.
H/D12: Knowing how to apply instrumental techniques and work protocols in a laboratory, applying regulations and strategies related to health and safety, waste management and quality.
Competences
Comp01: Students should be able to gather and interpret relevant data (usually within their area of study) to make judgements that include reflection on relevant social, scientific or ethical issues.
Comp02: Students are able, both in writing and orally, to discuss and convey information, ideas, problems and solutions related to Biotechnology to both specialist and non-specialist/general audiences.
Comp03: That students have developed those learning skills necessary to undertake further studies with a high degree of autonomy.
Comp04: That students know how to apply the theoretical-practical knowledge acquired in the degree in a professional manner and are competent in the approach/solution of problems, as well as in the elaboration/defence of arguments in both academic and professional contexts related to innovation and the biotechnology industry.
Comp05: Study and learn autonomously, with organised time and resources, new knowledge and techniques in Biotechnology and acquire the ability to work in a team.
The lecture classes will use the master class methodology with audiovisual support, emphasising the basic concepts of the subject, especially those more difficult to understand. Active methodologies will be used and student participation in teaching activities will be encouraged and valued. In the seminars, of compulsory attendance, students will carry out teamwork, which they will have to present and debate with the rest of their classmates. In the practical sessions, of compulsory attendance, students will carry out activities autonomously and in teams, under the supervision of the teaching staff and following the protocols/indications previously given. Each student's activity will be monitored (attendance, participation, elaboration and quality in the resolution of programmed activities, etc.). Students will have access, through the virtual classroom, to the essential information and material necessary for the development of the activities of the subject. There will be tutorials, both face-to-face and through the Microsoft Teams platform, to guide learning, as a complementary tool for the preparation of seminars/activities and to solve doubts about the subject. In addition, a public tutoring forum will be available in the virtual classroom for students to raise and solve doubts that may be of general interest to the rest of the students. Students will take a final test as part of their assessment (see next section) and will be able to choose the language in which to take it (Galician/Spanish).
There will be a continuous assessment that will represent up to 30% of the maximum overall grade, which will serve to assess the following learning outcomes: Con01, Con06, H/D02, H/D05, H/D10, H/D12, Comp01, Comp02, Comp03, Comp04 and Comp05. Continuous assessment will consist of the assessment of the work done in group seminars, class attendance, attitude and participation and the completion and content of the activities scheduled during the course, which may be face-to-face or virtual (synchronous or asynchronous). In addition, there will be a final test to assess the theoretical-practical knowledge of the subject, the following skills/skills and competences: Con01, Con06, H/D02, H/D12, Comp03, Comp04, Comp05. This exam will represent up to 70% of the overall maximum mark and it will be necessary for the student to achieve a minimum mark of 50% to pass it and to be able to weight the grade. This test will also be compulsory for students who do not complete the continuous assessment. Attendance in interactive classes is a prerequisite to pass the subject. The evaluation system will be the same in both opportunities of the call. Repeating students will keep the attendance to practical and the grade obtained in the continuous assessment, in case there is a record.
In the case of fraudulent exercises or tests, Regulations on the Evaluation of Students' Academic Performance and the Revision of Grades (Resolution of 15 June 2011, DOG of 21 July 2011) will apply.
4.5 ECTS credits:
Expository classes: 20h
Interactive laboratory classes: 10h
Interactive seminar classes: 7h
Tutorials in small groups: 1h
Individualized tutorials: 0.5h
Exams completion and review: 2h
Student's personal work: 72h
It is recommended that the student carries out an active learning of the knowledge that is acquired throughout the course, using schemes, and linking the already owned knowledge with the newly acquired. It is recommended to participate in class, to study the subject periodically and to use tutorials, to guide the work/study and to solve doubts. In addition, the student should consult the bibliography to solve doubts and complement the learning. It is important for the student to become aware of the importance of understanding the basis of the concepts and techniques to be worked on to develop skills for their application to real situations in their future jobs.
To get the most out of the course, it is recommended that students have previous knowledge of cell biology, English to handle the bibliography/teaching resources and skills in the use of information and communication technologies. Students will have access to the virtual classroom of the course on Moodle platform, with the resources and communication channels it offers. In addition, Microsoft Teams application and e-mail can be used for teacher-student communication.
Jannette Rodriguez Pallares
Coordinador/a- Department
- Morphological Science
- Area
- Human Anatomy and Embryology
- Phone
- 881815466
- jannette.rodriguez [at] usc.es
- Category
- Professor: University Lecturer
Juan Andres Parga Martin
- Department
- Morphological Science
- Area
- Human Anatomy and Embryology
- juan.parga [at] usc.es
- Category
- Professor: University Lecturer
Andrea Lopez Lopez
- Department
- Morphological Science
- Area
- Human Anatomy and Embryology
- andrealopez.lopez [at] usc.es
- Category
- Professor: Intern Assistant LOSU
Maria Del Carmen Diaz Ruiz
- Department
- Morphological Science
- Area
- Human Anatomy and Embryology
- Phone
- 881812463
- mdelcarmen.diaz [at] usc.es
- Category
- Professor: University Lecturer
| Monday | |||
|---|---|---|---|
| 09:00-10:00 | Grupo /CLE_01 | Spanish | Classroom 08. Louis Pasteur |
| Wednesday | |||
| 09:00-10:00 | Grupo /CLE_01 | Spanish | Classroom 08. Louis Pasteur |