ECTS credits ECTS credits: 6
ECTS Hours Rules/Memories Student's work ECTS: 99 Hours of tutorials: 3 Expository Class: 24 Interactive Classroom: 24 Total: 150
Use languages Spanish (62.24%), Galician (37.76%)
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Analytical Chemistry, Nutrition and Bromatology
Areas: Analytical Chemistry
Center Faculty of Biology
Call: Second Semester
Teaching: Sin docencia (Extinguida)
Enrolment: No Matriculable | 1st year (Yes)
1. To know and know how to apply the basic analytical and instrumental techniques useful in a Biotechnology laboratory.
2. Know how to describe the fundamentals and uses of spectrophotometry, microscopy, mass spectrometry, chromatography and electrophoresis.
3. Know how to identify and interpret spectra of various types for the structural identification of biomolecules and other applications
4. Know how to choose the right technique to solve each particular problem based on analytical criteria
5. Distinguish between chromatographic and electrophoretic separation techniques and select them appropriately for different bioseparation applications.
(Each topic will last for about 2 hours, except for topics 6 and 9 which will last for 3 hours).
Topic 1. Introduction to the basic techniques in Biotechnology
Topic 2. Introduction to spectroscopic methods and classification.
Topic 3. UV-Vis spectrophotometry
Topic 4. Fluorescence spectrophotometry and related techniques
Topic 5. Fundamentals of Nuclear Magnetic Resonance (NMR)
Topic 6. Mass spectrometry (MS)
Topic 7. Introduction to chromatographic separation techniques
Topic 8. Gas chromatography
Topic 9. Liquid chromatographies
Topic 10. Electrophoretic techniques
LAB CLASSES PROGRAM
Lab 1: Basic operations in the analytical laboratory
Lab 2. Applications of UV-Vis Spectrophotometry to Biotechnology
Lab 3: Chromatography Applications to Biotechnology
Lab 4: Applications of Mass Spectrometry and other techniques (NMR&X-ray difraction)to Biotechnology
SEMINARS
Six seminars (11 hours) will be held in small groups, in which exercises, problems and/or simulations of cases related to the contents of the subject will be proposed and solved. Active student participation is required.
TUTORIALS
The tutorials (individual or in groups) will be used to clarify doubts, provide information or guidance to students, as well as to learn about their progress in the acquisition of skills.
BASIC
---SUSAN R. MIKKELSEN. QUIMICA BIOANALITICA: METODOS Y TEORIA ANALITICA PARA EL LABORATORIO DE BIOLOGIA MOLECULAR, FARMACIA Y BIOQUIMICA, 2011 Editorial: UNIVERSIDAD DE BUENOS AIRES. ISBN: 9789502317694
---D. C. HARRIS. "Análisis Químico Cuantitativo". Editorial Reverté, 3ª ed. (6ª del inglés), Barcelona (2007), recomendado cap. 26 págs. 654-666.
---D.A. SKOOG; D.M. WEST; F.J. HOLLER; S.R. CROUCH. “Química Analítica”, Thomson .8ª Ed. Madrid. 2005, cap. 33 págs. 1016-1024.
In addition, throughout the course it will be recommended to visit specific websites with videos and materials of interest for a better understanding of the topics.
COMPLEMENTARY
• “Esenciais USC” Collection
----TÉCNICAS ANALÍTICAS E INSTRUMENTAIS: introdución ás técnicas espectroscópicas: espectro(foto)metría de absorción molecular UV/Vis.
Autoras: Marta Lores Aguín, Carmen García Jares (2019)
---TÉCNICAS ANALÍTICAS E INSTRUMENTAIS: introdución ás técnicas de separación cromatográficas. Autoras: Carmen García Jares, Marta Lores Aguín (2019)
Basic and general skills:
Those listed in the Degree in Biotechnology Report.
Cross-cutting skills
CT1 - Think in an integrated way and approach problems from different perspectives.
CT3 - Organize and plan your work.
CT4 - Interpret experimental results and identify consistent and inconsistent elements.
CT7 - Maintain an ethical commitment.
CT5 - Teamwork.
CT6 - Critically Reasoning
CT8 - Adaptation to new situations (resilience).
Specific competencies:
CE1 - Know how to make calculations, analyze data and interpret experimental results in the fields of Biotechnology.
CE3 - Know and know how to apply the instrumental techniques and the protocols of work in a laboratory, applying the regulations and techniques related to safety and hygiene, waste management and quality.
CE5 - Know how to obtain and interpret information from the main databases (biological, omic, bibliographic) and know how to use bioinformatics tools.
CE10 - Know the techniques that allow to determine the properties of proteins and to be able to analyze and manipulate their structure according to its application in Biotechnology.
---Master classes (on-site), in which the teacher will explain the concepts of the subject with the support of audiovisual and computer media; it can have different formats (theory, problems and/or general examples, general guidelines of the subject), promoting the participation of the students.
---Seminars (on-site), in small groups, in which theory applications, exercises, problems, case simulations, etc. are proposed and solved. Active participation of students is required.
---Practical laboratory classes (on-site),, in which the students, following the protocols prepared for this purpose, will handle the appropriate equipment and will solve practical questions.
---Tutorials (individual or group, on-site) to clarify doubts, provide information or guidance to students, as well as to learn about progress in the acquisition of skills.
Attendance at seminars, tutorials and laboratory practicals is required.
The evaluation will consist of two parts:
Ongoing assessment: Ongoing assessment will be done through oral and/or written tests, assignments submitted and/or presented, student participation in the classroom and in the laboratory, and tutorials. Next, the evaluation of each of the activities to be carried out by the student is indicated, in order to give the final grade, as well as the typology, methods and characteristics of the evaluation system of the subject:
1. Attendance at interactive small group classes (seminars), tutorials and lab practices is mandatory.
2. Non-attendance at a minimum of 80% of the joint hours of seminars and tutorials implies a waiver of the right to continuous assessment.
3. Attendance at all laboratory practices is mandatory, as well as passing the evaluation of these practices. Failure to attend will prevent the subject from being passed both in the ordinary opportunity and in the opportunity for recovery. In order to pass the course, the student must achieve the qualification of apt in laboratory practice.
Final exam (EF)
The final exam will cover all the contents of the course.
The different activities will count towards the student's final grade as follows:
Seminars 20% of the total
Lab 20%
Final Exam 60%
For the evaluation of laboratory practices, the following items will be evaluated:
Attitude and aptitude in the laboratory
Execution of the lab practice (including presentation of results)
Final report
Continuous assessment is complementary to the completion of the final exam. The student's grade will be the one obtained by weighting the final exam with the continuous assessment; although to have the option of weighting it is necessary to have a minimum grade of 4 out of 10 points in the final exam. The student's grade will not be lower than the final exam grade or the one obtained by weighting it with the continuous assessment.
All activities that are classified as compulsory will be assessable.
Skills Assessment : Seminars (S) Lab (P) Tutorials (T) Final Exam (EF)
CG1: S, P, T, EF
CG2: S, P, T, EF
CG3: S, P, T, EF
CG4: S, P, T, EF
CG5: S, P, T, EF
CB1: S, T, EF
CB2: S, T
CB3: S, P, T, EF
CB4: S, P, T
CB5: S, P, T, EF
CT1: S, P, T, EF
CT3: S, P
CT4: S, P
CT5: S, P, T
CT6: S, P, T, EF
CT7: S, P, T, EF
CT8: S, P, T, EF
CE1: S, P, EF
CE3: P
CE5: S, P
CE10: S, P
Re-students who have passed laboratory practices will have their grade retained for a maximum of two academic years. Therefore, they will not have to do the laboratory practices again, but they will attend the other interactive classes (seminars and tutorials) on equal terms with the other students.
Re-students who have passed the lab practices can repeat them again if they want. They just need to communicate to the professor-coordinator.
FACE-TO-FACE WORK IN THE CLASSROOM HOURS
Large Group Expositive Classes 22
Interactive small group classes (Seminars) 11
Interactive lessons with computer (small group) 00
Tutorials (very small group) 03
Laboratory practices 15
Total hours of classroom or laboratory work 51
STUDENT'S PERSONAL WORK HOURS
Independent individual or group study 44
Resolution of exercises or other work 22
Preparation of laboratory work and preparation of the lab report 30
Total hours of personal work of the student 96
It is advisable to attend the lectures and to use the recommended bibliography, and to carry out the exercises and work proposed.
With regard to the practical part of the course, it is recommended that you read the practice scripts before entering the laboratory.
Registered students need to know and obey the "normas xerais de seguridade nos laboratorios de prácticas" of the University of Santiago de Compostela, to participate in laboratory sessions. This regulation is in the webpage (www.usc.es/estaticos/servizos/sprl/normalumlab.pdf)
A virtual classroom on the Moodle platform (Virtual Campus of the USC, https://cv.usc.es/) will be available to students, which will serve as a repository of the material necessary for the correct development of the subject as well as a channel of communication with students. In addition, another direct communication channel may be operational through the MS-Teams application.
Carmen Maria Garcia Jares
- Department
- Analytical Chemistry, Nutrition and Bromatology
- Area
- Analytical Chemistry
- Phone
- 881814394
- carmen.garcia.jares [at] usc.es
- Category
- Professor: University Professor
Marta Lores Aguin
Coordinador/a- Department
- Analytical Chemistry, Nutrition and Bromatology
- Area
- Analytical Chemistry
- Phone
- 881814386
- marta.lores [at] usc.es
- Category
- Professor: University Professor
Aly Jesús Castillo Zamora
- Department
- Analytical Chemistry, Nutrition and Bromatology
- Area
- Analytical Chemistry
- alyjesus.castillo.zamora [at] usc.es
- Category
- Predoutoral_Doutoramento Industrial
| Monday | ||
|---|---|---|
| 09:00-10:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |
| Tuesday | ||
| 09:00-10:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |
| 05.23.2024 16:00-20:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |
| 07.11.2024 16:00-20:00 | Grupo /CLE_01 | Classroom 03. Carl Linnaeus |
| Teacher | Language |
|---|---|
| GARCIA JARES, CARMEN MARIA | Spanish |
| LORES AGUIN, MARTA | Galician |
| Teacher | Language |
|---|---|
| Castillo Zamora, Aly Jesús | Spanish |
| GARCIA JARES, CARMEN MARIA | Spanish |
| LORES AGUIN, MARTA | Galician |
| Teacher | Language |
|---|---|
| Castillo Zamora, Aly Jesús | Spanish |
| GARCIA JARES, CARMEN MARIA | Spanish |
| LORES AGUIN, MARTA | Galician |
| Teacher | Language |
|---|---|
| Castillo Zamora, Aly Jesús | Spanish |
| GARCIA JARES, CARMEN MARIA | Spanish |
| LORES AGUIN, MARTA | Galician |
| Teacher | Language |
|---|---|
| GARCIA JARES, CARMEN MARIA | Spanish |
| LORES AGUIN, MARTA | Galician |
| Teacher | Language |
|---|---|
| GARCIA JARES, CARMEN MARIA | Spanish |
| LORES AGUIN, MARTA | Galician |
| Teacher | Language |
|---|---|
| GARCIA JARES, CARMEN MARIA | Spanish |
| LORES AGUIN, MARTA | Galician |
| Teacher | Language |
|---|---|
| GARCIA JARES, CARMEN MARIA | Spanish |
| LORES AGUIN, MARTA | Galician |
| Teacher | Language |
|---|---|
| GARCIA JARES, CARMEN MARIA | Spanish |
| LORES AGUIN, MARTA | Galician |
| Teacher | Language |
|---|---|
| GARCIA JARES, CARMEN MARIA | Spanish |
| LORES AGUIN, MARTA | Galician |
| Teacher | Language |
|---|---|
| GARCIA JARES, CARMEN MARIA | Spanish |
| LORES AGUIN, MARTA | Galician |
| Teacher | Language |
|---|---|
| GARCIA JARES, CARMEN MARIA | Spanish |
| LORES AGUIN, MARTA | Galician |